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Posted on February 2, 2024
What kinds of positive controls are necessary for calcium assays?
Calcium IndicatorsIntracellular IonsPhysiological ProbesRatiometric Calcium Indicators
What kinds of positive controls are necessary for calcium assays?
Calcium IndicatorsIntracellular IonsPhysiological ProbesRatiometric Calcium Indicators
Posted on February 2, 2024
Are there any reasons for using flow cytometry to measure calcium flux?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Are there any reasons for using flow cytometry to measure calcium flux?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Posted on February 2, 2024
Can I use the same calcium reagents for microscopy as I do for flow?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsMicroscopyPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Can I use the same calcium reagents for microscopy as I do for flow?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsMicroscopyPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Posted on February 2, 2024
Is it possible to measure real time calcium flux with a flow cytometer?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Is it possible to measure real time calcium flux with a flow cytometer?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Posted on February 2, 2024
Can I measure what proportion of cells are responsive in my calcium flux assay?
Calcium IndicatorsEC50Intracellular IonsPhysiological ProbesRatiometric Calcium Indicators
Can I measure what proportion of cells are responsive in my calcium flux assay?
Calcium IndicatorsEC50Intracellular IonsPhysiological ProbesRatiometric Calcium Indicators
Posted on February 2, 2024
What are the advantages of using a ratiometric calcium probe vs. a single-channel dye?
Calcium IndicatorsIntracellular IonsPhysiological ProbesRatiometric Calcium Indicators
What are the advantages of using a ratiometric calcium probe vs. a single-channel dye?
Calcium IndicatorsIntracellular IonsPhysiological ProbesRatiometric Calcium Indicators
Posted on February 2, 2024
Which calcium dyes work with flow cytometry lasers?
Calcium IndicatorsFlow Cytometry ReagentsFluoresceinsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Which calcium dyes work with flow cytometry lasers?
Calcium IndicatorsFlow Cytometry ReagentsFluoresceinsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Posted on February 2, 2024
Can I combine Fura Red with Fluo-3 or Fluo-4 for ratiometric analysis?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Can I combine Fura Red with Fluo-3 or Fluo-4 for ratiometric analysis?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Posted on February 2, 2024
Should cells be equilibrated before a flow calcium assay?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Should cells be equilibrated before a flow calcium assay?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Posted on February 2, 2024
What kinds of stimulants are used for flow calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
What kinds of stimulants are used for flow calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Posted on February 2, 2024
What is the purpose of ionomycin in calcium flux flow assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
What is the purpose of ionomycin in calcium flux flow assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Posted on February 2, 2024
What buffers should be used for flow calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
What buffers should be used for flow calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Posted on February 2, 2024
What is the difference between negative control and baseline for flow cytometry calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
What is the difference between negative control and baseline for flow cytometry calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Posted on February 2, 2024
Are there any substitutes for probenecid in calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometryprobenecid
Are there any substitutes for probenecid in calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometryprobenecid
Posted on February 2, 2024
Is probenecid required for calcium assays?
Calcium IndicatorsIntracellular IonsPhysiological ProbesRatiometric Calcium Indicatorsprobenecid
Is probenecid required for calcium assays?
Calcium IndicatorsIntracellular IonsPhysiological ProbesRatiometric Calcium Indicatorsprobenecid
Posted on February 2, 2024
What is the purpose of probenecid in calcium flux flow assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometryprobenecid
What is the purpose of probenecid in calcium flux flow assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometryprobenecid
Posted on February 2, 2024
Is a wash step required for calcium flux flow assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Is a wash step required for calcium flux flow assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry
Posted on February 2, 2024
What is the dye concentration for calcium indicators?
Calcium IndicatorsIntracellular IonsPhysiological ProbesRatiometric Calcium Indicators
What is the dye concentration for calcium indicators?
Calcium IndicatorsIntracellular IonsPhysiological ProbesRatiometric Calcium Indicators
Posted on February 2, 2024
Are there any red calcium flux dyes?
Calcium IndicatorsIntracellular IonsPhysiological ProbesRatiometric Calcium Indicators
Are there any red calcium flux dyes?
Calcium IndicatorsIntracellular IonsPhysiological ProbesRatiometric Calcium Indicators
Posted on July 25, 2023
What is the role of calcium ions (Ca2+) as a second messenger?
Calcium IndicatorsCell SignalingCellular ProcessesIntracellular IonsPhysiological ProbesSecond Messenger
What is the role of calcium ions (Ca2+) as a second messenger?
Calcium IndicatorsCell SignalingCellular ProcessesIntracellular IonsPhysiological ProbesSecond Messenger
Posted on October 7, 2022
How do cells recognize signals?
Calcium IndicatorsChemical ReagentsIntracellular IonsPhysiological Probes
How do cells recognize signals?
Calcium IndicatorsChemical ReagentsIntracellular IonsPhysiological Probes
Posted on October 7, 2022
What are the similarities between intracellular and intercellular signaling?
Calcium IndicatorsIntracellular IonsIntracellular pHPhysiological Probes
What are the similarities between intracellular and intercellular signaling?
Calcium IndicatorsIntracellular IonsIntracellular pHPhysiological Probes
Posted on July 29, 2021
What is ratiometric imaging?
Ratiometric Calcium IndicatorsCalcium IndicatorsPhysiological ProbesIntracellular Ions
What is ratiometric imaging?
Ratiometric Calcium IndicatorsCalcium IndicatorsPhysiological ProbesIntracellular Ions
Posted on July 29, 2021
How does calcium imaging work?
Cell SignalingCalcium IndicatorsPhysiological Probes
How does calcium imaging work?
Cell SignalingCalcium IndicatorsPhysiological Probes
Posted on July 29, 2021
Why is Fura-2 unable to cross cell membranes?
Ratiometric Calcium IndicatorsCalcium IndicatorsIntracellular IonsPhysiological Probes
Why is Fura-2 unable to cross cell membranes?
Ratiometric Calcium IndicatorsCalcium IndicatorsIntracellular IonsPhysiological Probes
Posted on July 29, 2021
What is 2-photon calcium imaging?
Ratiometric Calcium IndicatorsCalcium IndicatorsIntracellular Ions
What is 2-photon calcium imaging?
Ratiometric Calcium IndicatorsCalcium IndicatorsIntracellular Ions
Posted on July 29, 2021
How does Fura red work?
Ratiometric Calcium IndicatorsCalcium IndicatorsCell Signaling
How does Fura red work?
Ratiometric Calcium IndicatorsCalcium IndicatorsCell Signaling
Posted on July 28, 2021
What does calcium imaging measure?
Calcium IndicatorsCell SignalingIntracellular Ions
What does calcium imaging measure?
Calcium IndicatorsCell SignalingIntracellular Ions
Posted on July 26, 2021
What kind of microscope do I need for ratiometric dyes?
Ratiometric Calcium IndicatorsCalcium IndicatorsIntracellular IonsPhysiological Probes
What kind of microscope do I need for ratiometric dyes?
Ratiometric Calcium IndicatorsCalcium IndicatorsIntracellular IonsPhysiological Probes
Posted on July 23, 2021
What is the difference between Fura-2 and Quin-2
Calcium IndicatorsIntracellular IonsRatiometric Calcium Indicators
What is the difference between Fura-2 and Quin-2
Calcium IndicatorsIntracellular IonsRatiometric Calcium Indicators
Posted on July 23, 2021
What is a dual excitation dye?
Calcium IndicatorsRatiometric Calcium IndicatorsPhysiological Probes
What is a dual excitation dye?
Calcium IndicatorsRatiometric Calcium IndicatorsPhysiological Probes
Posted on July 23, 2021
What is the difference between ratiometric imaging and calcium imaging?
Calcium IndicatorsRatiometric Calcium IndicatorsPhysiological Probes
What is the difference between ratiometric imaging and calcium imaging?
Calcium IndicatorsRatiometric Calcium IndicatorsPhysiological Probes
Posted on July 23, 2021
What is a dual-wavelength ion indicator?
Ratiometric Calcium IndicatorsCalcium IndicatorsPhysiological Probes
What is a dual-wavelength ion indicator?
Ratiometric Calcium IndicatorsCalcium IndicatorsPhysiological Probes
Posted on July 23, 2021
What are ratiometric dyes?
Ratiometric Calcium IndicatorsCalcium IndicatorsPhysiological Probes
What are ratiometric dyes?
Ratiometric Calcium IndicatorsCalcium IndicatorsPhysiological Probes
Posted on July 23, 2021
What is a ratiometric indicator?
Ratiometric Calcium IndicatorsCalcium IndicatorsPhysiological Probes
What is a ratiometric indicator?
Ratiometric Calcium IndicatorsCalcium IndicatorsPhysiological Probes
Posted on July 23, 2021
What is ratiometric fluorescence used to detect?
Ratiometric Calcium IndicatorsCalcium IndicatorsPhysiological Probes
What is ratiometric fluorescence used to detect?
Ratiometric Calcium IndicatorsCalcium IndicatorsPhysiological Probes
Posted on July 23, 2021
What is the difference between calcium imaging and electrophysiology?
Calcium IndicatorsIntracellular IonsPhysiological Probes
What is the difference between calcium imaging and electrophysiology?
Calcium IndicatorsIntracellular IonsPhysiological Probes
Posted on July 22, 2021
How does the increase in intracellular calcium cause contraction?
Endoplasmic Reticulum (ER)Calcium IndicatorsG-Protein-Coupled Receptors (GPCR)
How does the increase in intracellular calcium cause contraction?
Endoplasmic Reticulum (ER)Calcium IndicatorsG-Protein-Coupled Receptors (GPCR)
Posted on July 22, 2021
What regulates calcium concentration?
Cell SignalingCalcium IndicatorsG-Protein-Coupled Receptors (GPCR)
What regulates calcium concentration?
Cell SignalingCalcium IndicatorsG-Protein-Coupled Receptors (GPCR)
Posted on July 22, 2021
What is a calcium flux assay?
Calcium IndicatorsCell SignalingIntracellular IonsPhysiological Probes
What is a calcium flux assay?
Calcium IndicatorsCell SignalingIntracellular IonsPhysiological Probes
Posted on July 21, 2021
Where is intracellular calcium stored?
Endoplasmic Reticulum (ER)Calcium Indicators
Where is intracellular calcium stored?
Endoplasmic Reticulum (ER)Calcium Indicators
Posted on July 21, 2021
How is intracellular calcium measured?
Calcium IndicatorsCell SignalingIntracellular IonsPhysiological Probes
How is intracellular calcium measured?
Calcium IndicatorsCell SignalingIntracellular IonsPhysiological Probes
Posted on February 3, 2021
What is the relationship between concentration and absorbance in spectrophotometry?
Calcium IndicatorsPhysiological Probes
What is the relationship between concentration and absorbance in spectrophotometry?
Calcium IndicatorsPhysiological Probes
Posted on January 22, 2021
What affect does magnesium have on neurotransmitter release?
Cell MetabolismCalcium IndicatorsCellular Processes
What affect does magnesium have on neurotransmitter release?
Cell MetabolismCalcium IndicatorsCellular Processes
Posted on January 20, 2021
How does calcium cause exocytosis?
Intracellular IonsCalcium IndicatorsCell Signaling
How does calcium cause exocytosis?
Intracellular IonsCalcium IndicatorsCell Signaling
Posted on November 30, 2020
How is calcium used as a second messenger?
Calcium IndicatorsIntracellular IonsPhysiological Probes
How is calcium used as a second messenger?
Calcium IndicatorsIntracellular IonsPhysiological Probes